キタダ　マサアキ   KITADA MASAAKI   北田　容章    所属   関西医科大学  解剖学講座    職種   教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Comparison of separation methods for tissue-derived extracellular vesicles in the liver, heart, and skeletal muscle
掲載誌名	正式名：FEBS open bio 略　 称：FEBS Open Bio ISSNコード：22115463
掲載区分	国外
巻・号・頁	11(2),pp.482-493
著者・共著者	Matejovič A, Wakao S, Kitada M, Kushida Y, Dezawa M
発行年月	2021/02
概要	Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation-based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV-related markers, with minimum levels of intracellular organelle-related markers. Some tissue-specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs.
DOI	10.1002/2211-5463.13075
PMID	33410274