キムラ　トシヤ   KIMURA TOSHIYA   木村　俊哉    所属   関西医科大学  iPS・幹細胞応用医学講座    職種   助教
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	CAMSAP3 is required for mTORC1-dependent ependymal cell growth and lateral ventricle shaping in mouse brains.
掲載誌名	正式名：Development ISSNコード：14779129/09501991
出版社	The Company of Biologists
巻・号・頁	148(3),pp.dev195073
著者・共著者	Toshiya Kimura, Hiroko Saito, Miwa Kawasaki, Masatoshi Takeichi
担当区分	筆頭著者,責任著者
発行年月	2021/02
概要	Microtubules (MTs) regulate numerous cellular processes, but their roles in brain morphogenesis are not well known. Here, we show that CAMSAP3, a non-centrosomal microtubule regulator, is important for shaping the lateral ventricles. In differentiating ependymal cells, CAMSAP3 became concentrated at the apical domains, serving to generate MT networks at these sites. Camsap3-mutated mice showed abnormally narrow lateral ventricles, in which excessive stenosis or fusion was induced, leading to a decrease of neural stem cells at the ventricular and subventricular zones. This defect was ascribed at least in part to a failure of neocortical ependymal cells to broaden their apical domain, a process necessary for expanding the ventricular cavities. mTORC1 was required for ependymal cell growth but its activity was downregulated in mutant cells. Lysosomes, which mediate mTORC1 activation, tended to be reduced at the apical regions of the mutant cells, along with disorganized apical MT networks at the corresponding sites. These findings suggest that CAMSAP3 supports mTORC1 signaling required for ependymal cell growth via MT network regulation, and, in turn, shaping of the lateral ventricles.
DOI	10.1242/dev.195073
PMID	33462112