ハットリ フミユキ
HATTORI FUMIYUKI 服部 文幸 所属 関西医科大学 iPS・幹細胞再生医学講座 職種 研究教授 |
|
論文種別 | 原著(症例報告除く) |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | "All-in-one"in vitro selection of collagen-binding vascular endothelial growth factor. |
掲載誌名 | 正式名:Biomaterials 略 称:Biomaterials ISSNコード:1878590501429612 |
掲載区分 | 国外 |
巻・号・頁 | 161,pp.270-278 |
著者・共著者 | Park SH, Uzawa T, Hattori F, Ogino S, Morimoto N, Tsuneda S, Ito Y. |
発行年月 | 2018/04 |
概要 | To enhance the therapeutic effect of growth factors, a powerful strategy is to direct their localization to damaged sites. To treat skin wounds and myocardial infarction, we selected vascular endothelial growth factor (VEGF) carrying binding affinity to collagen. A simple conjugation of a reported collagen-binding sequence and VEGF did not increase the collagen-binding affinity, indicating that the molecular interaction between the two proteins abolished collagen binding activity. Here, we present a new molecular evolution strategy,"all-in-one"in vitro selection, in which a collagen-binding VEGF (CB-VEGF) was directly identified from a random library consisting of random and VEGF sequences. As expected, the selected CB-VEGFs exhibited high binding affinity to collagen and maintained the same growth enhancement activity for endothelial cells as unmodified VEGF in solution. Furthermore, the selected CB-VEGF enhanced angiogenesis at skin wounds and infarcted myocardium. This study demonstrates that"all-in-one"in vitro selection is a novel strategy for the design of functional proteins for regenerative medicine. |
DOI | 10.1016/j.biomaterials.2018.01.055 |
PMID | 29425847 |