ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Analysis of cardiomyocyte movement in the developing murine heart.
掲載誌名	正式名：Biochemical and biophysical research communications 略　 称：Biochem Biophys Res Commun ISSNコード：109021040006291X
巻・号・頁	464(4),pp.1000-1007
著者・共著者	Hashimoto Hisayuki, Yuasa Shinsuke, Tabata Hidenori, Tohyama Shugo, Seki Tomohisa, Egashira Toru, Hayashiji Nozomi, Hattori Fumiyuki, Kusumoto Dai, Kunitomi Akira, Takei Makoto, Kashimura Shin, Yozu Gakuto, Shimojima Masaya, Motoda Chikaaki, Muraoka Naoto, Nakajima Kazunori, Sakaue-Sawano Asako, Miyawaki Atsushi, Fukuda Keiichi
発行年月	2015/09
概要	The precise assemblage of several types of cardiac precursors controls heart organogenesis. The cardiac precursors show dynamic movement during early development and then form the complicated heart structure. However, cardiomyocyte movements inside the newly organized mammalian heart remain unclear. We previously established the method of ex vivo time-lapse imaging of the murine heart to study cardiomyocyte behavior by using the Fucci (fluorescent ubiquitination-based cell cycle indicator) system, which can effectively label individual G1, S/G2/M, and G1/S-transition phase nuclei in living cardiomyocytes as red, green, and yellow, respectively. Global analysis of gene expression in Fucci green positive ventricular cardiomyocytes confirmed that cell cycle regulatory genes expressed in G1/S, S, G2/M, and M phase transitions were upregulated. Interestingly, pathway analysis revealed that many genes related to the cell cycle were significantly upregulated in the Fucci green positive ventricular cardiomyocytes, while only a small number of genes related to cell motility were upregulated. Time-lapse imaging showed that murine proliferating cardiomyocytes did not exhibit dynamic movement inside the heart, but stayed on site after entering the cell cycle.
DOI	10.1016/j.bbrc.2015.07.036
PMID	26168730