ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Time-lapse imaging of cell cycle dynamics during development in living cardiomyocyte.
掲載誌名	正式名：Journal of molecular and cellular cardiology 略　 称：J Mol Cell Cardiol ISSNコード：1095858400222828
巻・号・頁	72,pp.241-9
著者・共著者	Hashimoto Hisayuki, Yuasa Shinsuke, Tabata Hidenori, Tohyama Shugo, Hayashiji Nozomi, Hattori Fumiyuki, Muraoka Naoto, Egashira Toru, Okata Shinichiro, Yae Kojiro, Seki Tomohisa, Nishiyama Takahiko, Nakajima Kazunori, Sakaue-Sawano Asako, Miyawaki Atsushi, Fukuda Keiichi
発行年月	2014/07
概要	Mammalian cardiomyocytes withdraw from the cell cycle shortly after birth, although it remains unclear how cardiomyocyte cell cycles behave during development. Compared to conventional immunohistochemistry in static observation, time-lapse imaging can reveal comprehensive data in hard-to-understand biological phenomenon. However, there are no reports of an established protocol of successful time-lapse imaging in mammalian heart. Thus, it is valuable to establish a time-lapse imaging system to enable the observation of cell cycle dynamics in living murine cardiomyocytes. This study sought to establish time-lapse imaging of murine heart to study cardiomyocyte cell cycle behavior. The Fucci (fluorescent ubiquitination-based cell cycle indicator) system can effectively label individual G1, S/G2/M, and G1/S-transition phase nuclei red, green and yellow, respectively, in living mammalian cells, and could therefore be useful to visualize the real-time cell cycle transitions in living murine heart. To establish a similar system for time-lapse imaging of murine heart, we first developed an ex vivo culture system, with the culture conditions determined in terms of sample state, serum concentration, and oxygen concentration. The optimal condition (slice culture, oxygen concentration 20%, serum concentration 10%) successfully mimicked physiological cardiomyocyte proliferation in vivo. Time-lapse imaging of cardiac slices from E11.5, E14.5, E18.5, and P1 Fucci-expressing transgenic mice revealed an elongated S/G2/M phase in cardiomyocytes during development. Our time-lapse imaging of murine heart revealed a gradual elongation of the S/G2/M phase during development in living cardiomyocytes.
DOI	10.1016/j.yjmcc.2014.03.020
PMID	24704900