ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes.
掲載誌名	正式名：Cell stem cell 略　 称：Cell Stem Cell ISSNコード：1875977718759777
巻・号・頁	12(1),pp.127-37
著者・共著者	Tohyama Shugo, Hattori Fumiyuki, Sano Motoaki, Hishiki Takako, Nagahata Yoshiko, Matsuura Tomomi, Hashimoto Hisayuki, Suzuki Tomoyuki, Yamashita Hiromi, Satoh Yusuke, Egashira Toru, Seki Tomohisa, Muraoka Naoto, Yamakawa Hiroyuki, Ohgino Yasuyuki, Tanaka Tomofumi, Yoichi Masatoshi, Yuasa Shinsuke, Murata Mitsushige, Suematsu Makoto, Fukuda Keiichi
発行年月	2013/01
概要	Heart disease remains a major cause of death despite advances in medical technology. Heart-regenerative therapy that uses pluripotent stem cells (PSCs) is a potentially promising strategy for patients with heart disease, but the inability to generate highly purified cardiomyocytes in sufficient quantities has been a barrier to realizing this potential. Here, we report a nongenetic method for mass-producing cardiomyocytes from mouse and human PSC derivatives that is based on the marked biochemical differences in glucose and lactate metabolism between cardiomyocytes and noncardiomyocytes, including undifferentiated cells. We cultured PSC derivatives with glucose-depleted culture medium containing abundant lactate and found that only cardiomyocytes survived. Using this approach, we obtained cardiomyocytes of up to 99% purity that did not form tumors after transplantation. We believe that our technological method broadens the range of potential applications for purified PSC-derived cardiomyocytes and could facilitate progress toward PSC-based cardiac regenerative therapy.
DOI	10.1016/j.stem.2012.09.013
PMID	23168164