ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation.
掲載誌名	正式名：The Journal of experimental medicine 略　 称：J Exp Med ISSNコード：1540953800221007
巻・号・頁	208(4),pp.715-27
著者・共著者	Hara Mie, Yuasa Shinsuke, Shimoji Kenichiro, Onizuka Takeshi, Hayashiji Nozomi, Ohno Yohei, Arai Takahide, Hattori Fumiyuki, Kaneda Ruri, Kimura Kensuke, Makino Shinji, Sano Motoaki, Fukuda Keiichi
発行年月	2011/04
概要	After skeletal muscle injury, neutrophils, monocytes, and macrophages infiltrate the damaged area; this is followed by rapid proliferation of myoblasts derived from muscle stem cells (also called satellite cells). Although it is known that inflammation triggers skeletal muscle regeneration, the underlying molecular mechanisms remain incompletely understood. In this study, we show that granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is expressed in developing somites. G-CSFR and G-CSF were expressed in myoblasts of mouse embryos during the midgestational stage but not in mature myocytes. Furthermore, G-CSFR was specifically but transiently expressed in regenerating myocytes present in injured adult mouse skeletal muscle. Neutralization of endogenous G-CSF with a blocking antibody impaired the regeneration process, whereas exogenous G-CSF supported muscle regeneration by promoting the proliferation of regenerating myoblasts. Furthermore, muscle regeneration was markedly impaired in G-CSFR-knockout mice. These findings indicate that G-CSF is crucial for skeletal myocyte development and regeneration and demonstrate the importance of inflammation-mediated induction of muscle regeneration.
DOI	10.1084/jem.20101059
PMID	21422169