|
ハットリ フミユキ
HATTORI FUMIYUKI 服部 文幸 所属 関西医科大学 iPS・幹細胞再生医学講座 職種 研究教授 |
|
| 論文種別 | 原著(症例報告除く) |
| 言語種別 | 英語 |
| 査読の有無 | その他(不明) |
| 表題 | In vitro pharmacologic testing using human induced pluripotent stem cell-derived cardiomyocytes. |
| 掲載誌名 | 正式名：Biochemical and biophysical research communications 略 称：Biochem Biophys Res Commun ISSNコード：109021040006291X |
| 巻・号・頁 | 385(4),pp.497-502 |
| 著者・共著者 | Tanaka Tomofumi, Tohyama Shugo, Murata Mitsushige, Nomura Fumimasa, Kaneko Tomoyuki, Chen Hao, Hattori Fumiyuki, Egashira Toru, Seki Tomohisa, Ohno Yohei, Koshimizu Uichi, Yuasa Shinsuke, Ogawa Satoshi, Yamanaka Shinya, Yasuda Kenji, Fukuda Keiichi |
| 発行年月 | 2009/08 |
| 概要 | The lethal ventricular arrhythmia Torsade de pointes (TdP) is the most common reason for the withdrawal or restricted use of many cardiovascular and non-cardiovascular drugs. The lack of an in vitro model to detect pro-arrhythmic effects on human heart cells hinders the development of new drugs. We hypothesized that recently established human induced pluripotent stem (hiPS) cells could be used in an in vitro drug screening model. In this study, hiPS cells were driven to differentiate into functional cardiomyocytes, which expressed cardiac markers including Nkx2.5, GATA4, and atrial natriuretic peptide. The hiPS-derived cardiomyocytes (hiPS-CMs) were analyzed using a multi electrode assay. The application of ion channel inhibitors resulted in dose-dependent changes to the field potential waveform, and these changes were identical to those induced in the native cardiomyocytes. This study shows that hiPS-CMs represent a promising in vitro model for cardiac electrophysiologic studies and drug screening. |
| DOI | 10.1016/j.bbrc.2009.05.073 |
| PMID | 19464263 |