ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Common marmoset embryonic stem cell can differentiate into cardiomyocytes.
掲載誌名	正式名：Biochemical and biophysical research communications 略　 称：Biochem Biophys Res Commun ISSNコード：109021040006291X
巻・号・頁	369(3),pp.801-6
著者・共著者	Chen Hao, Hattori Fumiyuki, Murata Mitsushige, Li Weizhen, Yuasa Shinsuke, Onizuka Takeshi, Shimoji Kenichiro, Ohno Yohei, Sasaki Erika, Kimura Kensuke, Hakuno Daihiko, Sano Motoaki, Makino Shinji, Ogawa Satoshi, Fukuda Keiichi
発行年月	2008/05
概要	Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessedcharacteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.
DOI	10.1016/j.bbrc.2008.02.141
PMID	18331825