ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	E-cadherin-coated plates maintain pluripotent ES cells without colony formation.
掲載誌名	正式名：PloS one 略　 称：PLoS One ISSNコード：1932620319326203
巻・号・頁	1,pp.e15
著者・共著者	Nagaoka Masato, Koshimizu Uichi, Yuasa Shinsuke, Hattori Fumiyuki, Chen Hao, Tanaka Tomofumi, Okabe Masaru, Fukuda Keiichi, Akaike Toshihiro
発行年月	2006/12
概要	Embryonic stem (ES) cells cultured on gelatin-coated plates or feeder layers form tight aggregated colonies by the E-cadherin-mediated cell-cell adhesions. Here we show that murine ES cells do not make cell-cell contacts or form colonies when cultured on the plate coated with a fusion protein of E-cadherin and IgG Fc domain. The cells in culture retain all ES cell features including pluripotency to differentiate into cells of all three germ layers and germ-line transmission after extended culture. Furthermore, they show a higher proliferative ability, lower dependency on LIF, and higher transfection efficiency than colony-forming conditions. Our results suggest that aggregated colony formation might inhibit diffusion of soluble factors and increase cell-cell communication, which may result in a heterogeneous environment within and between surrounding cells of the colony. This method should enable more efficient and scalable culture of ES cells, an important step towards the clinical application of these cells.
DOI	10.1371/journal.pone.0000015
PMID	17183641