マツウラ トオル   MATSUURA TORU
  松浦 徹
   所属   関西医科大学  病理学講座
   職種   講師
論文種別 原著(症例報告除く)
言語種別 英語
査読の有無 査読あり
表題 DNA Replication Is Required for Circadian Clock Function by Regulating Rhythmic Nucleosome Composition
掲載誌名 正式名:Molecular Cell
略  称:Mol Cell
ISSNコード:1097-2765
掲載区分国外
巻・号・頁 67(2),203-213頁
著者・共著者 Xiao Liu, Yunkun Dang, Toru Matsu-ura, Yubo He, Qun He, Christian Hong, Yi Liu
発行年月 2017/07
概要 Although the coupling between circadian and cell cycles allows circadian clocks to gate cell division and DNA replication in many organisms, circadian clocks were thought to function independently of cell cycle. Here, we show that DNA replication is required for circadian clock function in Neurospora. Genetic and pharmacological inhibition of DNA replication abolished both overt and molecular rhythmicities by repressing frequency (frq) gene transcription. DNA replication is essential for the rhythmic changes of nucleosome composition at the frq promoter. The FACT complex, known to be involved in histone disassembly/reassembly, is required for clock function and is recruited to the frq promoter in a replication-dependent manner to promote replacement of histone H2A.Z by H2A. Finally, deletion of H2A.Z uncoupled the dependence of the circadian clock on DNA replication. Together, these results establish circadian clock and cell cycle as interdependent coupled oscillators and identify DNA replication as a critical process in the circadian mechanism.