マツウラ トオル   MATSUURA TORU
  松浦 徹
   所属   関西医科大学  病理学講座
   職種   講師
論文種別 原著(症例報告除く)
言語種別 英語
査読の有無 査読あり
表題 Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture
掲載誌名 正式名:Molecular Cell
略  称:Mol Cell
ISSNコード:1097-2765
掲載区分国外
巻・号・頁 64(5),900-912頁
著者・共著者 Toru Matsu-ura, Andrey Dovzhenok, Eitaro Aihara, Jill Rood, Yan Ren, Tongli Zhang, Marshall H. Montrose, Sookkyung Lim, Sean R. Moore, Christian I. Hong
発行年月 2016/12
概要 Circadian clock-gated cell division cycles are observed from cyanobacteria to mammals via intracellular molecular connections between these two oscillators. Here we demonstrate WNT-mediated intercellular coupling between the cell cycle and circadian clock in 3D murine intestinal organoids (enteroids). The circadian clock gates a population of cells with heterogeneous cell-cycle times that emerge as 12-hr synchronized cell division cycles. Remarkably, we observe reduced-amplitude oscillations of circadian rhythms in intestinal stem cells and progenitor cells, indicating an intercellular signal arising from differentiated cells governing circadian clock-dependent synchronized cell division cycles. Stochastic simulations and experimental validations reveal Paneth cell-secreted WNT as the key intercellular coupling component linking the circadian clock and cell cycle in enteroids.