マツウラ トオル   MATSUURA TORU
  松浦 徹
   所属   関西医科大学  病理学講座
   職種   講師
論文種別 原著(症例報告除く)
言語種別 英語
査読の有無 査読あり
表題 Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid
掲載誌名 正式名:Scientific Reports
略  称:Sci Rep
ISSNコード:2045-2322
巻・号・頁 5(17185),doi: 10.1038/srep17185頁
著者・共著者 Eitaro Aihara, Maxime MAHE, Michael Schumacher, Andrea Matthis, Rui Feng, Wenwen Ren, Taeko Noah, Toru Matsu-ura, Sean Moore, Christian Hong, Yana Zavros, Scott Herness, Noah Shroyer, Ken Iwatsuki, Peihua Jiang, Michael Helmrath, Marshall Montrose
発行年月 2015/11
概要 Leucine-rich repeat-containing G-protein coupled receptor 5-expressing (Lgr5(+)) cells have been identified as stem/progenitor cells in the circumvallate papillae, and single cultured Lgr5(+) cells give rise to taste cells. Here we use circumvallate papilla tissue to establish a three-dimensional culture system (taste bud organoids) that develops phenotypic characteristics similar to native tissue, including a multilayered epithelium containing stem/progenitor in the outer layers and taste cells in the inner layers. Furthermore, characterization of the cell cycle of the taste bud progenitor niche reveals striking dynamics of taste bud development and regeneration. Using this taste bud organoid culture system and FUCCI2 transgenic mice, we identify the stem/progenitor cells have at least 5 distinct cell cycle populations by tracking within 24-hour synchronized oscillations of proliferation. Additionally, we demonstrate that stem/progenitor cells have motility to form taste bud organoids. Taste bud organoids provides a system for elucidating mechanisms of taste signaling, disease modeling, and taste tissue regeneration.