マツウラ　トオル   MATSUURA TORU   松浦　徹    所属   関西医科大学  病理学講座    職種   講師
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells
掲載誌名	正式名：Journal of Cell Biology 略　 称：J Cell Biol ISSNコード：15408140
掲載区分	国外
巻・号・頁	173(5),pp.755-765
著者・共著者	Toru Matsu-ura, Takayuki Michikawa, Takafumi Inoue, Atsushi Miyawaki, Manabu Yoshida, Katsuhiko Mikoshiba
担当区分	筆頭著者
発行年月	2006/06
概要	We developed genetically encoded fluorescent inositol 1,4,5-trisphosphate (IP3) sensors that do not severely interfere with intracellular Ca2+ dynamics and used them to monitor the spatiotemporal dynamics of both cytosolic IP3 and Ca2+ in single HeLa cells after stimulation of exogenously expressed metabotropic glutamate receptor 5a or endogenous histamine receptors. IP3 started to increase at a relatively constant rate before the pacemaker Ca2+ rise, and the subsequent abrupt Ca2+ rise was not accompanied by any acceleration in the rate of increase in IP3. Cytosolic [IP3] did not return to its basal level during the intervals between Ca2+ spikes, and IP3 gradually accumulated in the cytosol with a little or no fluctuations during cytosolic Ca2+ oscillations. These results indicate that the Ca2+ -induced regenerative IP3 production is not a driving force of the upstroke of Ca2+ spikes and that the apparent IP3 sensitivity for Ca2+ spike generation progressively decreases during Ca2+ oscillations.