ウエムラ　ヨシコ   UEMURA YOSHIKO   植村　芳子    所属   関西医科大学  病理学講座    職種   学長特命教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Immunohistochemical features of the human retina and retinoblastoma.
掲載誌名	正式名：Virchows Archiv 略　 称：Virchows Arch ISSNコード：09456317
巻・号・頁	426(6),pp.571-575
著者・共著者	Yuge K, Nakajima M, Uemura Y, Miki H, Uyama M, Tsubura A.
発行年月	1995/04
概要	The immunohistochemical features of 24 retinoblastoma specimens from 22 patients, 15 with unilateral and 7 with bilateral disease, were examined by the labelled streptavidin biotin (LSAB) method and compared with those of specimens from the remaining morphologically normal retina. In the normal retina, S-100 protein, glial fibrillary acidic protein (GFAP) and vimentin were detected in astrocytes and/or Müller cells. Neurofilament protein was seen in axons of the ganglion cells, synaptophysin was present in both plexiform layers, bcl-2 oncoprotein was seen in ganglion cells and bipolar cells, and neuron-specific enolase (NSE) was detected in ganglion cells, bipolar cells and photoreceptor cells and in their cell processes. While retinoblastoma (Rb) protein expression was noted in ganglion cells, bipolar cells, and some photoreceptor cells, p53 protein was not expressed at all. In all retinoblastomas, strong NSE expression and weak bcl-2 expression was observed in almost all tumour cells and synaptophysin was localized in rosette-forming cells, while tumour cells were devoid of S-100, GFAP, vimentin and neurofilament protein. These findings support the view that retinoblastomas are composed of neuron-committed cells. In addition, no Rb protein expression was detected in retinoblastomas, whereas p53 expression was found in 18 cases (75%).