マツウラ　トオル   MATSUURA TORU   松浦　徹    所属   関西医科大学  病理学講座    職種   講師
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Synthetic gene network with positive feedback loop amplifies cellulase gene expression in Neurospora crassa
掲載誌名	正式名：ACS Synthetic Biology 略　 称：ACS Synth Biol ISSNコード：2161-5063
掲載区分	国外
巻・号・頁	7(5),pp.1395-1405
著者・共著者	Toru Matsu-ura, Andrey Dovzhenok, Samuel T. Coradetti, Krithika R. Subramanian, Daniel R. Meyer, Jaesang J. Kwon, Caleb Kim, Nathan Salomonis, N. Louise Glass, Sookkyung Lim, Christian I. Hong
担当区分	筆頭著者,責任著者
発行年月	2018/05
概要	Second-generation or lignocellulosic biofuels are a tangible source of renewable energy, which is critical to combat climate change by reducing the carbon footprint. Filamentous fungi secrete cellulose-degrading enzymes called cellulases, which are used for production of lignocellulosic biofuels. However, inefficient production of cellulases is a major obstacle for industrial-scale production of second-generation biofuels. We used computational simulations to design and implement synthetic positive feedback loops to increase gene expression of a key transcription factor, CLR-2, that activates a large number of cellulases in a filamentous fungus, Neurospora crassa. Overexpression of CLR-2 reveals previously unappreciated roles of CLR-2 in lignocellulosic gene network, which enabled simultaneous induction of approximately 50% of 78 lignocellulosic degradation-related genes in our engineered Neurospora strains. This engineering results in dramatically increased cellulase activity due to cooperative orchestration of multiple enzymes involved in the cellulose degradation pathway. Our work provides a proof of principle in utilizing mathematical modeling and synthetic biology to improve the efficiency of cellulase synthesis for second-generation biofuel production.
DOI	10.1021/acssynbio.8b00011
PMID	29625007