マツウラ　トオル   MATSUURA TORU   松浦　徹    所属   関西医科大学  病理学講座    職種   講師
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano
掲載誌名	正式名：Nature Methods 略　 称：Nat Methods ISSNコード：15487091
掲載区分	国外
巻・号・頁	7(9),pp.729-732
著者・共著者	Kazuki Horikawa, Yoshiyuki Yamada, Tomoki Matsuda, Kentaro Kobayashi, Mitsuhiro Hashimoto, Toru Matsu-ura, Atsushi Miyawaki, Takayuki Michikawa, Katsuhiko Mikoshiba, Takeharu Nagai
発行年月	2010/09
概要	We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.