マツウラ　トオル   MATSUURA TORU   松浦　徹    所属   関西医科大学  病理学講座    職種   講師
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Seizure-mediated neuronal activation induces DREAM gene expression in the mouse brain
掲載誌名	正式名：Molecular Brain Research 略　 称：Brain Res Mol Brain Res ISSNコード：0169328X
掲載区分	国外
巻・号・頁	109(1-2),pp.198-206
著者・共著者	Toru Matsu-ura, Yoshiyuki Konishi, Tsutomu Aoki, Jose R. Naranjo, Katsuhiko Mikoshiba, Taka-aki Tamura
担当区分	筆頭著者
発行年月	2002/12
概要	Various transcriptional activators are induced in neurons concomitantly with long-lasting neural activity, whereas only a few transcription factors are known to act as neural activity-inducible transcription repressors. In this study, mRNA of DREAM (DRE-antagonizing modulator), a Ca(2+)-modulated transcriptional repressor, was demonstrated to accumulate in the mouse brain after pentylenetetrazol (PTZ)-induced seizures. Accumulation in the mouse hippocampus reached maximal level in the late phase (at 7-8 h) after PTZ injection. Kainic acid induced the same response. Interestingly, the late induction of DREAM expression required new protein synthesis and was blocked by MK801 suggesting that Ca(2+)-influx via NMDA receptors is necessary for the PTZ-mediated DREAM expression. In situ hybridization revealed that PTZ-induced DREAM mRNA accumulation was observed particularly in the dentate gyrus, cerebral cortex, and piriform cortex. The results of the present study demonstrate that DREAM is a neural activity-stimulated late gene and suggest its involvement in adaptation to long-lasting neuronal activity.