ノナカ　マサヒロ   NONAKA MASAHIRO   埜中　正博    所属   関西医科大学  脳神経外科学講座    職種   教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Kainic acid-induced seizure upregulates Na(+)/myo-inositol cotransporter mRNA in rat brain.
掲載誌名	正式名：Brain research. Molecular brain research 略　 称：Brain Res Mol Brain Res ISSNコード：0169328X0169328X
掲載区分	国外
巻・号・頁	70(2),pp.179-186
著者・共著者	Nonaka M, Kohmura E, Yamashita T, Yamauchi A, Fujinaka T, Yoshimine T, Tohyama M, Hayakawa T
担当区分	筆頭著者
発行年月	1999/07
概要	A major organic osmolyte, myo-inositol protects cells from perturbing effects of high intracellular concentrations of electrolytes. Myo-inositol is accumulated into cells through Na(+)/myo-inositol cotransporter (SMIT). In order to investigate the regulation of SMIT in generalized seizure, we employed Northern blot analysis and in situ hybridization to study the changes in SMIT mRNA expression in kainic acid-injected rats. Northern blot analysis demonstrated that SMIT mRNA began to increase in the brain 2 h after onset of seizure, and peaked at 12 h. In situ hybridization revealed rapid increase of SMIT mRNA (2 h of seizure) in the CA3 hippocampal pyramidal cells and in the dentate granular cells. Then, at 4-6 h SMIT mRNA expression was observed in the other limbic structure such as amygdala and piriform cortex. Finally, in neocortex and in CA1 pyramidal cells, SMIT mRNA was slowly increased and peaked at 12 h. Microautoradiogram demonstrated that cells expressed SMIT mRNA were mainly neurons. These results suggest that SMIT mRNA is upregulated by kainic acid-induced seizure primarily in structures involved in seizure activity.
PMID	10407166