ヤマダ　ヒサオ   YAMADA HISAO   山田　久夫    所属   関西医科大学  専門部    職種   名誉教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	AUF1, an mRNA decay factor, has a discordant role in Cpeb1 expression
掲載誌名	正式名：Biochemical and biophysical research communications 略　 称：Biochem Biophys Res Commun ISSNコード：0006291X/10902104
掲載区分	国外
巻・号・頁	534,pp.491-497
著者・共著者	Oe S, Koike T, Hirahara Y, Tanaka S, Hayashi S, Nakano Y, Kase M, Noda Y, Yamada H, Kitada M
発行年月	2021/01
概要	Cytoplasmic polyadenylation element binding protein 1 (CPEB1) regulates polyadenylation and subsequent translation of CPE-containing mRNAs involved in various physiological and pathological phenomena. Although the significance of CPEB1-mediated translational regulation has recently been reported, the detailed regulatory mechanism of Cpeb1 expression remains unclear. To elucidate the post-transcriptional regulatory mechanisms of Cpeb1 expression, we constructed reporter plasmids containing various deletions or mutations in the Cpeb1 mRNA 3' untranslated region (3'UTR). We investigated their expression levels in Neuro2a neuroblastoma cells. We found that Cpeb1 expression is regulated through an AU-rich element in its 3'UTR. Furthermore, the mRNA decay factor AU-rich binding factor 1 (AUF1) regulates Cpeb1 expression, and knockdown of AUF1 upregulates Cpeb1 mRNA expression but results in a decrease in CPEB1 protein levels. These findings indicate that AUF1 has a discordant role in the expression of Cpeb1.
DOI	10.1016/j.bbrc.2020.11.054
PMID	33220927