ヒラハラ　ユキエ   HIRAHARA YUKIE   平原　幸恵    所属   関西医科大学  基礎看護学領域    職種   教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	Scaffold attachment factor B: distribution and interaction with ERα in the rat brain
掲載誌名	正式名：Histochemistry and cell biology 略　 称：Histochem Cell Biol ISSNコード：09486143/1432119X
掲載区分	国外
巻・号・頁	153(5),pp.323-338
著者・共著者	Hashimoto T, Kawata M, Hirahara Y, Nishi M, Satoshi I, Matsuda K
発行年月	2020/05
概要	Scaffold attachment factor (SAFB) 1 and its homologue SAFB2 are multifunctional proteins that are involved in various cellular mechanisms, including chromatin organization and transcriptional regulation, and are also corepressors of estrogen receptor alpha (ERα). Both SAFBs are expressed at high levels in the brain. However, the distributions of SAFB1 and SAFB2 have yet to be characterized in detail and it is unclear whether both proteins interact with ERα in the brain. In this study, we investigated the expression and distribution of both SAFBs and their interaction with ERα in adult male rat brain. Immunohistochemical staining showed that SAFB1 and SAFB2 have a similar distribution pattern and are widely expressed throughout the brain. Double-fluorescence immunohistochemical and immunocytochemical analyses in primary cultures showed that the two SAFB proteins are localized in nuclei of neurons, astrocytes, and oligodendrocytes. Of note, SAFB2 was also found in cytoplasmic regions in these cell lineages. Both SAFB proteins were also expressed in ERα-positive cells in the medial preoptic area (MPOA) and arcuate and ventromedial hypothalamic nuclei. Co-immunoprecipitation experiments revealed that both SAFB proteins from the MPOA reciprocally interact with endogenous ERα. These results indicate that, in addition to a role in basal cellular function in the brain, the SAFB proteins may serve as ERα corepressors in hormone-sensitive regions.
DOI	10.1007/s00418-020-01853-1
PMID	32086573