ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	査読あり
表題	"All-in-one"in vitro selection of collagen-binding vascular endothelial growth factor.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：1878590501429612
掲載区分	国外
巻・号・頁	161,pp.270-278
著者・共著者	Park SH, Uzawa T, Hattori F, Ogino S, Morimoto N, Tsuneda S, Ito Y.
発行年月	2018/04
概要	To enhance the therapeutic effect of growth factors, a powerful strategy is to direct their localization to damaged sites. To treat skin wounds and myocardial infarction, we selected vascular endothelial growth factor (VEGF) carrying binding affinity to collagen. A simple conjugation of a reported collagen-binding sequence and VEGF did not increase the collagen-binding affinity, indicating that the molecular interaction between the two proteins abolished collagen binding activity. Here, we present a new molecular evolution strategy,"all-in-one"in vitro selection, in which a collagen-binding VEGF (CB-VEGF) was directly identified from a random library consisting of random and VEGF sequences. As expected, the selected CB-VEGFs exhibited high binding affinity to collagen and maintained the same growth enhancement activity for endothelial cells as unmodified VEGF in solution. Furthermore, the selected CB-VEGF enhanced angiogenesis at skin wounds and infarcted myocardium. This study demonstrates that"all-in-one"in vitro selection is a novel strategy for the design of functional proteins for regenerative medicine.
DOI	10.1016/j.biomaterials.2018.01.055
PMID	29425847