ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.
掲載誌名	正式名：Cell stem cell 略　 称：Cell Stem Cell ISSNコード：1875977718759777
巻・号・頁	6(3),pp.227-37
著者・共著者	Shimoji Kenichiro, Yuasa Shinsuke, Onizuka Takeshi, Hattori Fumiyuki, Tanaka Tomofumi, Hara Mie, Ohno Yohei, Chen Hao, Egasgira Toru, Seki Tomohisa, Yae Kojiro, Koshimizu Uichi, Ogawa Satoshi, Fukuda Keiichi
発行年月	2010/03
概要	During a screen for humoral factors that promote cardiomyocyte differentiation from embryonic stem cells (ESCs), we found marked elevation of granulocyte colony-stimulating factor receptor (G-CSFR) mRNA in developing cardiomyocytes. We confirmed that both G-CSFR and G-CSF were specifically expressed in embryonic mouse heart at the midgestational stage, and expression levels were maintained throughout embryogenesis. Intrauterine G-CSF administration induced embryonic cardiomyocyte proliferation and caused hyperplasia. In contrast, approximately 50% of csf3r(-/-) mice died during late embryogenesis because of the thinning of atrioventricular walls. ESC-derived developing cardiomyocytes also strongly expressed G-CSFR. When extrinsic G-CSF was administered to the ESC- and human iPSC-derived cardiomyocytes, it markedly augmented their proliferation. Moreover, G-CSF-neutralizing antibody inhibited their proliferation. These findings indicated that G-CSF is critically involved in cardiomyocyte proliferation during development, and may be used to boost the yield of cardiomyocytes from ESCs for their potential application to regenerative medicine.
DOI	10.1016/j.stem.2010.01.002
PMID	20207226