ハットリ　フミユキ   HATTORI FUMIYUKI   服部　文幸    所属   関西医科大学  iPS・幹細胞再生医学講座    職種   研究教授
論文種別	原著(症例報告除く)
言語種別	英語
査読の有無	その他(不明)
表題	Nongenetic method for purifying stem cell-derived cardiomyocytes.
掲載誌名	正式名：Nature methods 略　 称：Nat Methods ISSNコード：1548710515487091
巻・号・頁	7(1),pp.61-6
著者・共著者	Hattori Fumiyuki, Chen Hao, Yamashita Hiromi, Tohyama Shugo, Satoh Yu-Suke, Yuasa Shinsuke, Li Weizhen, Yamakawa Hiroyuki, Tanaka Tomofumi, Onitsuka Takeshi, Shimoji Kenichiro, Ohno Yohei, Egashira Toru, Kaneda Ruri, Murata Mitsushige, Hidaka Kyoko, Morisaki Takayuki, Sasaki Erika, Suzuki Takeshi, Sano Motoaki, Makino Shinji, Oikawa Shinzo, Fukuda Keiichi
発行年月	2010/01
概要	Several applications of pluripotent stem cell (PSC)-derived cardiomyocytes require elimination of undifferentiated cells. A major limitation for cardiomyocyte purification is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamine methyl ester perchlorate, could be used to selectively mark embryonic and neonatal rat cardiomyocytes, as well as mouse, marmoset and human PSC-derived cardiomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardiomyocytes transplanted into testes did not induce teratoma formation. Moreover, aggregate formation of PSC-derived cardiomyocytes through homophilic cell-cell adhesion improved their survival in the immunodeficient mouse heart. Our approaches will aid in the future success of using PSC-derived cardiomyocytes for basic and clinical applications.
DOI	10.1038/nmeth.1403
PMID	19946277