タカハシ　カンジ   TAKAHASHI KANJI   髙橋　寛二    所属   関西医科大学  眼科学講座    職種   非常勤講師
言語種別	英語
発表タイトル	Combination Method of Magnetic Beads and Magnet Helps Sustain Number of Donor Bone Marrow Cells after Intra-Bone Marrow Injection, Resulting in Rapid Hemopoietic Recovery マグネットビーズとマグネットを用いた骨髄内骨髄移植の造血回復における効果
会議名	Japanese　Society for Immunity 第39回日本免疫学会総会学術集会
学会区分	全国規模の学会
発表形式	ポスター掲示
発表者・共同発表者	◎Shima C, Adachi Y, Shi Ming,Yanai S, Imai Y, Takahashi K, Ikehara S
発表年月日	2009/12
開催地 （都市, 国名）	大阪
概要	We have developed a new intra-bone marrow-bone marrow transplantation (IBM-BMT)method, in which donor bone marrow cells (BMCs) are directly injected into the recipient’s bone marrow (BM), resulting in a rapid recovery of donor hemopoiesis and a reduction in the severity of GVHD. In the present experiment, we attempted to retain the injected BMCs inside the BM using magnetic beads and a magnet. The BMCs of donor mice were conjugated with magnetic beads, and these cells were then injected into the BM of recipient mice with a magnet (magnet-IBM group) and compared with conventional IBM-BMT without a magnet (IBM group). A significantly higher number of transplanted cells were detected in the injected BM in the magnet-IBM group. We next carried out day-12 CFU-S assays to examine the early stage of the hemopoiesis of the injected host hemopoietic stem cells after IBM-BMT. The spleens of mice in the magnet-IBM group showed considerably higher CFU-S counts than those in the IBM group. Excellent reconstitution of donor hemopoietic cells in the magnet-IBM group was observed 1 month after IBM-BMT. These results suggest that the IBM-BMT using the combination of magnetic beads and a magnet is superior to the conventional IBM-BMT.