タカハシ　カンジ   TAKAHASHI KANJI   髙橋　寛二    所属   関西医科大学  眼科学講座    職種   非常勤講師
言語種別	英語
発表タイトル	A new histological evaluation to detectresidual OVDs in cataract surgery
会議名	Asia ARVO2015
発表者・共同発表者	◎Mori H, Yamada H, Takahashi K
発表年月日	2015/02
開催地 （都市, 国名）	Yokohama, Japan
概要	Purpose To establish a new hitstological evaluation to detect residual ophthalmic viscosurgical devices(OVDs) and its adhesion to the corneal endothelium in phacoemulsification and aspiration(PEA) surgery. Methods In this study, we conducted research by using adult postmortem porcine eyes. Viscoat®, HealonV®, Healon® and DiscoVisc® were used as OVDs. Each OVD was mixed with fluorescent conjugated dextrans to make it visible. All surgery were performed by one surgeon(H.M). Surgical procedures were as follows: After making a corneal side port, a continuous curvilinear capsulorhexis was conducted under the water irrigation. Then, corneal tunnel incision was made and the anterior capsule was removed. Each single OVD was injectioned by 0.4cc through a corneal side port. The lens was removed using single-hand phacomulsification technique. After the lens was completely removed, ultrasound oscillation was done for ten seconds in anterior chamber to simulate human eye with hard lens nucleus. After operation, the anterior segment was isolated by equatorial incision and the tissue was frozen by shimmering liquid nitrogen. Twenty micrometers thin-sliced sections were made by Cryostat from limbus to limbus by sagital manner. Each one out of ten slides from all sectioned slides(700 sections in each eye) were picked up and dried naturally at room temperature and were photographed by a fluorescent microscopy with cool CCD camera(Olympus, Tokyo, Japan,) immediately. Using WinRoof®(Mitani shoji, Tokyo, Japan,) software, we measured the percentage of OVDs which cover corneal endothelium and the volume of OVD clots remaining in the anterior chamber.