タケトウ　メグミ   TAKETOU MEGUMI   武藤　恵    所属   関西医科大学  生理学講座    職種   講師
言語種別	英語
発表タイトル	Function of metabotropic glutamate receptors in the marginal zone of neonatal rat hippocampi
会議名	95th Annual Meeting of the Physiological Society of Japan
主催者	the Physiological Society of Japen
学会区分	全国規模の学会
発表形式	ポスター掲示
講演区分	一般
発表者・共同発表者	◎Megumi Taketo, Hiroko Matsuda
発表年月日	2018/03/28
開催地 （都市, 国名）	Takamatsu, Kagawa, Japan
概要	Type I metabotropic glutamate receptors (mGluRs) are 7 transmembrane-spanning receptors which couple to Gq protein and cause intracellular Ca2+ mobilization. Two type I mGluR subtypes, mGluR1 and mGluR5, are detected in central nervous system. These receptors regulate neuronal excitability and concern with synaptic plasticity. Hippocampal marginal zone contains early-generated neurons including Cajal-Retzius cells (C-R cells), which play an important role in migration of neurons through secretion of glycoprotein, reelin. Though function of secreted reelin has been well studied, less is known about regulation of C-R cell excitability. In the present experiments, characteristics of the Ca2+ mobilization by activation of mGluR1 in hippocampal marginal zone were determined. Ca2+ imaging was performed using acute slices of neonatal rat hippocampus. Elevation of intracellular calcium concentration ([Ca2+]i) was induced by glutamate application in C-R cells, and this elevation was not prevented by blockade of ionotropic glutamate receptors. Type 1 mGluR-specific agonist increased [Ca2+]i in the presence of mGluR5 specific antagonist. Contrarily, mGluR1-specific antagonist prevented the [Ca2+]i increment induced by type I agonist. These results demonstrate that neonatal hippocampal C-R cells express functional mGluR1. Effects of application of other excitatory neurotransmitters on [Ca2+]i were also measured and compared with that of mGluR agonist in mGluR1-expressing neurons of hippocampal marginal zone.