フジイ　チヒロ   FUJII CHIHIRO   藤井　ちひろ    所属   関西医科大学  神経内科学講座    職種   講師
言語種別	英語
発表タイトル	Myelin glycolipid sulfatide alters B cell functions: Roles in the pathogenesis of multiple sclerosis
会議名	第62回日本神経学会学術大会
学会区分	全国規模の学会
発表形式	ポスター掲示
講演区分	一般
発表者・共同発表者	◎濱谷美緒, 越智博文, 木村公俊, 髙田真基, 錦織隆成, 芦田真士, 藤井ちひろ, 川村和之, 水野敏樹, 髙橋良輔, 上野英樹, 近藤誉之
発表年月日	2021/05/21
開催地 （都市, 国名）	京都
概要	[OBJECTIVE] Recent evidence suggests critical pathogenic roles of B cells in multiple sclerosis (MS). The aim of this study was to examine how B cells respond to sulfatide, a major myelin-glycolipid that is robustly released at demyelinating sites of inflamed lesions of MS. [METHODS] Human peripheral blood B cells from five healthy participants were cultured with sulfatide and analyzed for phenotypic changes, cytokine secretion, and immunoglobulin production. [RESULTS] B cells exposed to sulfatide upregulated the expression of activated lymphocyte function-associated antigen-1 and C-X-C chemokine receptor type 5, molecules associated with B cell migration into the central nervous system (CNS). Treatment with sulfatide also promoted B cells to proliferate and produce immunoglobulin G (IgG). Furthermore, treated B cells enhanced the secretion of inflammatory cytokines including granulocyte-macrophage colony-stimulating factor together with an increase of co-stimulatory molecules. Our results suggest that sulfatide promotes the B cell infiltration and migration toward MS lesions or B cell aggregates in meninges of progressive MS. Exposure to sulfatide promoted B cells to produce IgG as well as to become more functional antigen-presenting cells. Potentiated secretion of IgG might be associated with oligoclonal band or high IgG index in MS cerebrospinal fluid. [CONCLUSION] Treatment with sulfatide caused phenotypic and functional alterations in B cells similar to those found in patients with MS. Thus, sulfatide might contribute to the MS pathogenesis by modulating B cell functions.