|
ミキ カツアキ
MIKI KATSUAKI 三木 克朗 所属 関西医科大学 眼科学講座 職種 診療講師 |
|
| 言語種別 | 英語 |
| 発表タイトル | Combination Antioxidant Therapy for Retinitis Pigmentosa (RP) |
| 会議名 | Association for Research in Vision and Ophthalmology 2011 Annual Meeting |
| 学会区分 | 国際学会及び海外の学会 |
| 発表者・共同発表者 | ◎Miki k, Miki A, Usui S, Peter A campochiaro |
| 発表年月日 | 2011/05 |
| 開催地 （都市, 国名） |
Fort Lauderdale, Florida,USA |
| 概要 | Purpose: To identify an optimal antioxidant regimen for treatment of RP.
Methods: Rd10+/+ mice were given drinking water supplemented with 5mg/ml dextromethorphan (DM, an NADPH oxidase inhibitor), 2.5 mg/ml phenyl-n-tert-butylnitrone (PBN), 7 mg/ml N-acetylcysteine (NAC), or all three between postnatal day (P) 21 and P50. Retinal function was assessed by electroretinograms (ERGs), rod survival was assessed by measurement of outer nuclear layer (ONL) thickness at P35, and cone survival was determined by measuring cone cell density in peanut agglutinin-stained retinal whole mounts at P50. Oxidative damage was assessed by ELISA for carbonyl adducts on proteins at P35. Results: At P50, mean (±SEM) photopic ERG b-wave amplitudes (μV) were significantly greater inrd10+/+ mice treated with DM (16.6±1.3, p<0.05), PBN (17.9±2.0, p<0.05), NAC (21.2±1.3, p<0.05), or DM+PBN+NAC (38.1±1.3, p<0.01) compared to untreated rd10+/+ mice (11.3±1.2). Mean cone density (cones/0.0529mm2) was also increased in rd10+/+ mice treated with DM (72±2, p<0.05), PBN (73±2, p<0.05), NAC (128±3, p<0.05), or DM+PBN+NAC (140±4, p<0.01) compared to untreated rd10+/+ mice (58±2). Each of the regimens reduced carbonyl adducts an indicator of oxidative damage. Conclusions: DM, PBN, and NAC provide additive benefit for maintenance of cone function and survival in a model of RP supporting consideration of this regimen for clinical trials. |