シマ　チエコ   SHIMA CHIEKO   嶋　千絵子    所属   関西医科大学  眼科学講座    職種   講師
言語種別	英語
発表タイトル	Combination Method of Magnetic Beads and Magnet Helps Sustain Number of Donor Bone Marrow Cells after Intra-Bone Marrow Injection, Resulting in Rapid Hemopoietic Recovery マグネットビーズとマグネットを用いた骨髄内骨髄移植の造血回復における効果
会議名	Japanese　Society for Immunity 第39回日本免疫学会総会学術集会
学会区分	全国規模の学会
発表形式	ポスター掲示
発表者・共同発表者	◎Shima C, Adachi Y, Shi Ming,Yanai S, Imai Y, Takahashi K, Ikehara S
発表年月日	2009/12
開催地 （都市, 国名）	大阪
概要	We have developed a new intra-bone marrow-bone marrow transplantation (IBM-BMT)method, in which donor bone marrow cells (BMCs) are directly injected into the recipient’s bone marrow (BM), resulting in a rapid recovery of donor hemopoiesis and a reduction in the severity of GVHD. In the present experiment, we attempted to retain the injected BMCs inside the BM using magnetic beads and a magnet. The BMCs of donor mice were conjugated with magnetic beads, and these cells were then injected into the BM of recipient mice with a magnet (magnet-IBM group) and compared with conventional IBM-BMT without a magnet (IBM group). A significantly higher number of transplanted cells were detected in the injected BM in the magnet-IBM group. We next carried out day-12 CFU-S assays to examine the early stage of the hemopoiesis of the injected host hemopoietic stem cells after IBM-BMT. The spleens of mice in the magnet-IBM group showed considerably higher CFU-S counts than those in the IBM group. Excellent reconstitution of donor hemopoietic cells in the magnet-IBM group was observed 1 month after IBM-BMT. These results suggest that the IBM-BMT using the combination of magnetic beads and a magnet is superior to the conventional IBM-BMT.